Fig 1: miR-130b-5p expression and RASAL1 mRNA expression in seven gastric cell lines. Expression of miR-130b-5p and RASAL1 at the mRNA level was evaluated by reverse transcription-quantitative polymerase chain reaction. GES-1 cells were used as the control. (A) miR-130b-5p expression in MGC803 cells and BGC823 cells was higher compared with the rest of the gastric cell lines studied. (B) RASAL1 mRNA expression was lower in MGC803 cells compared with other cell lines. *P<0.05. miR, microRNA; RASAL1, RAS protein activator like 1.
Fig 2: Effect of miR-130b-5p on RASAL1. (A) Luciferase reporter gene assay indicated lower RLU values in cells co-transfected with miR-130b-5p mimics (with concentration of 50 nM) and pGL3-RASAL1 (with concentration of 50 nM) in comparison with cells in other three groups (cells co-transfected with miR-130b-5p and psiC-2, cells co-transfected with NC and pGL3-RASAL1, and cells co-transfected with NC and psiC-2) (psiC-2 was used as control plasmid with concentration of 50 nM). (B) Reverse transcription-quantitative polymerase chain reaction analysis of mRNA expression of RASAL1 in cells transfected with miR-130b-5p mimics and miR-130b-5p inhibitor. (C) Western blot analysis of protein expression of RASAL1 in cells transfected with miR-130b-5p mimics and miR-130b-5p inhibitor. *P<0.05. miR, microRNA; RASAL1, RAS protein activator like 1; NC, negative control.
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